Detection of complement protein mRNA in human astrocytes by the polymerase chain reaction.

@article{Gordon1992DetectionOC,
  title={Detection of complement protein mRNA in human astrocytes by the polymerase chain reaction.},
  author={David Lee Gordon and V. M. Avery and D L Adrian and Tania A. Sadlon},
  journal={Journal of neuroscience methods},
  year={1992},
  volume={45 3},
  pages={191-7}
}
Primer sets specific for complement proteins, C3, factor B and factor I, were designed and used to amplify cDNA from cultured human astrocyte mRNA by the polymerase chain reaction. Appropriately sized PCR products of 506 bp, 885 bp and 146 bp, respectively, were generated and specificity was confirmed with Southern blotting using an enhanced… CONTINUE READING