Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation

@article{Ronai2007DetectionOC,
  title={Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation},
  author={Diana Ronai and Maria Dolores Iglesias-Ussel and Manxia Fan and Ziqiang Li and Alberto Pesquera Martin and Matthew D. Scharff},
  journal={The Journal of Experimental Medicine},
  year={2007},
  volume={204},
  pages={181 - 190}
}
After encounter with antigen, the antibody repertoire is shaped by somatic hypermutation (SHM), which leads to an increase in the affinity of antibodies for the antigen, and class-switch recombination (CSR), which results in a change in the effector function of antibodies. Both SHM and CSR are initiated by activation-induced cytidine deaminase (AID), which deaminates deoxycytidine to deoxyuridine in single-stranded DNA (ssDNA). The precise mechanism responsible for the formation of ssDNA in V… CONTINUE READING