Detection of cellular sialic acid content using nitrobenzoxadiazole carbonyl-reactive chromophores.

@article{Key2012DetectionOC,
  title={Detection of cellular sialic acid content using nitrobenzoxadiazole carbonyl-reactive chromophores.},
  author={Jessie A. Key and Caishun Li and Christopher W. Cairo},
  journal={Bioconjugate chemistry},
  year={2012},
  volume={23 3},
  pages={
          363-71
        }
}
The selective ligation of hydrazine and amino-oxy compounds with carbonyls has gained popularity as a detection strategy with the recognition of aniline catalysis as a way to accelerate the labeling reaction in water. Aldehydes are a convenient functional group choice since there are few native aldehydes found at the cell surface. Aldehydes can be selectively introduced into sialic acid containing glycoproteins by treatment with dilute sodium periodate. Thus, the combination of periodate… CONTINUE READING

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