Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase.

Abstract

In this study, we report an electrochemical microbial biosensor that was made by immobilizing a bacterial laccase on the surface of Escherichia coli cells followed by adsorption of modified live cells onto a glassy-carbon electrode. Expression and surface localization of laccase on target cells were confirmed by Western blotting, flow cytometry assays and… (More)
DOI: 10.1016/j.aca.2018.01.008

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