Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization

@article{Mcclintock2007DetectionOC,
  title={Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization},
  author={J. T. Mcclintock and I J Chan and S R Thaker and Albine Katial and Floyd E. Taub and Amy E. Aotaki-Keen and Leonard M. Hjelmeland},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
  year={2007},
  volume={28},
  pages={102-108}
}
SummaryUsing in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto-oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the… Expand
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Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: Regulation by TGF‐β
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References

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Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis
TLDR
The results establish that normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. Expand
Specific receptors for platelet-derived growth factor on cells derived from connective tissue and glia.
TLDR
It was concluded that PDGF did not share receptors with these factors and was found on skin fibroblasts, normal and malignant glial cells, smooth muscle cells, and 3T3 cells but not on epithelial-derived cells, neuroblastoma cells, endothelial cells, or peripheral lymphocytes. Expand
cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines
The amino-acid sequence of the precursor of the human tumour cell line-derived platelet-derived growth factor (PDGF) A-chain has been deduced from complementary DNA clones and the gene localized to… Expand
Subcellular localization of bFGF in human retinal pigment epithelium in vitro.
TLDR
Investigation of subcellular fractions by Western blot analysis indicated cytosolic as well as nuclear localization of the basic fibroblast growth factor, however, it was demonstrated that the higher molecular weight forms ofbasic fibro Blast growth factor predominate in the nucleus. Expand
Platelet-derived growth factor in chemotactic for fibroblasts
TLDR
PDGF could have two functions in wound healing: to attract fibroblasts to migrate into the clot and then to induce their proliferation, and to stimulate chemotaxis rather than random motility. Expand
Type beta transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval.
TLDR
The data show that this molecule is a growth factor for certain mesenchymal cells in monolayer culture but only after a prereplicative phase that is significantly longer than that of other growth factors. Expand
Platelet‐derived growth factor is a chemoattractant for vascular smooth muscle cells
TLDR
It is established that platelet‐derived growth factor (PDGF) acts as a true chemoattractant for cultured aortic smooth muscle cells, and that methylation reactions are required for the chemotactic response. Expand
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TLDR
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The c‐sis gene encodes a precursor of the B chain of platelet‐derived growth factor.
TLDR
Results suggest that c‐sis encodes a polypeptide precursor of the B chain, which could be encoded by sequences which lie 5′ to the five regions of v‐sis homology in c‐tic, or at a separate locus. Expand
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TLDR
Hydrophobic high-performance liquid chromatography of reduced and alkylated 125I-labeled platelet-derived growth factor resulted in the separation of two distinct radioactive components, leading to inhomogeneity in the intact PDGF molecule. Expand
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