Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization
@article{Mcclintock2007DetectionOC, title={Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization}, author={J. T. Mcclintock and I J Chan and S R Thaker and Albine Katial and Floyd E. Taub and Amy E. Aotaki-Keen and Leonard M. Hjelmeland}, journal={In Vitro Cellular \& Developmental Biology - Animal}, year={2007}, volume={28}, pages={102-108} }
SummaryUsing in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto-oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the…
4 Citations
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: Regulation by TGF‐β
- Biology, MedicineJournal of cellular physiology
- 2005
It is demonstrated that TGF‐β activated RPE cells produce PDGF that may act on fibroblasts and other mesenchyme derived cells which express PDGF receptors, which may facilitate the formation of fibrovascular tissues associated with PVR.
Tumor Stem Cells: How to Define Them and How to Find Them?
- Medicine, Biology
- 2008
This review will summarize the investigated surface markers of haematological and solid tumors’ stem cells since the appropriate identification and isolation of this particular subpopulation of tumor cells will be essential for developing more effective cancer treatments.
Chapter 10 Tumor Stem Cells: How to Define Them and How to Find Them?
- Biology, Medicine
- 2008
This review will summarize the investigated surface markers of haematological and solid tumors' stem cells since the appropriate identification and isolation of this particular subpopulation of tumor cells will be essential for developing more effec- tive cancer treatments.
New Technologies and Tissue Repair and Regeneration (2): Other Biotherapeutic Technologies
- BiologyRegenerative Medicine in China
- 2021
References
SHOWING 1-10 OF 21 REFERENCES
Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis
- BiologyNature
- 1985
The results establish that normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.
Specific receptors for platelet-derived growth factor on cells derived from connective tissue and glia.
- Biology, MedicineProceedings of the National Academy of Sciences of the United States of America
- 1981
It was concluded that PDGF did not share receptors with these factors and was found on skin fibroblasts, normal and malignant glial cells, smooth muscle cells, and 3T3 cells but not on epithelial-derived cells, neuroblastoma cells, endothelial cells, or peripheral lymphocytes.
cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines
- BiologyNature
- 1986
The amino-acid sequence of the precursor of the human tumour cell line-derived platelet-derived growth factor (PDGF) A-chain has been deduced from complementary DNA clones and the gene localized to…
Subcellular localization of bFGF in human retinal pigment epithelium in vitro.
- Biology, MedicineExperimental eye research
- 1992
Platelet-derived growth factor in chemotactic for fibroblasts
- Biology, MedicineThe Journal of cell biology
- 1982
PDGF could have two functions in wound healing: to attract fibroblasts to migrate into the clot and then to induce their proliferation, and to stimulate chemotaxis rather than random motility.
Type beta transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1985
The data show that this molecule is a growth factor for certain mesenchymal cells in monolayer culture but only after a prereplicative phase that is significantly longer than that of other growth factors.
Platelet‐derived growth factor is a chemoattractant for vascular smooth muscle cells
- BiologyJournal of cellular physiology
- 1982
It is established that platelet‐derived growth factor (PDGF) acts as a true chemoattractant for cultured aortic smooth muscle cells, and that methylation reactions are required for the chemotactic response.
Primary culture of human retinal glia.
- BiologyInvestigative ophthalmology & visual science
- 1991
It is indicated that the primary cultures arose principally from glial cells of the human retina but did not precisely identify the cell of origin.
The c‐sis gene encodes a precursor of the B chain of platelet‐derived growth factor.
- BiologyThe EMBO journal
- 1984
Results suggest that c‐sis encodes a polypeptide precursor of the B chain, which could be encoded by sequences which lie 5′ to the five regions of v‐sis homology in c‐tic, or at a separate locus.
Platelet-derived growth factor: identification of constituent polypeptide chains.
- ChemistryBiochemical and biophysical research communications
- 1982