Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization

@article{Mcclintock2007DetectionOC,
  title={Detection of c-Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization},
  author={J. T. Mcclintock and I J Chan and S R Thaker and Albine Katial and Floyd E. Taub and Amy E. Aotaki-Keen and Leonard M. Hjelmeland},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
  year={2007},
  volume={28},
  pages={102-108}
}
SummaryUsing in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto-oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the… Expand
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: Regulation by TGF‐β
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The results establish that normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. Expand
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It was concluded that PDGF did not share receptors with these factors and was found on skin fibroblasts, normal and malignant glial cells, smooth muscle cells, and 3T3 cells but not on epithelial-derived cells, neuroblastoma cells, endothelial cells, or peripheral lymphocytes. Expand
cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines
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Investigation of subcellular fractions by Western blot analysis indicated cytosolic as well as nuclear localization of the basic fibroblast growth factor, however, it was demonstrated that the higher molecular weight forms ofbasic fibro Blast growth factor predominate in the nucleus. Expand
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PDGF could have two functions in wound healing: to attract fibroblasts to migrate into the clot and then to induce their proliferation, and to stimulate chemotaxis rather than random motility. Expand
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TLDR
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