Detection of bphAa gene expression of Rhodococcus sp. strain RHA1 in soil using a new method of RNA preparation from soil.

@article{Wang2008DetectionOB,
  title={Detection of bphAa gene expression of Rhodococcus sp. strain RHA1 in soil using a new method of RNA preparation from soil.},
  author={Yong Wang and Jun Shimodaira and Tomomichi Miyasaka and Sho Morimoto and Takanori Oomori and Naoto Ogawa and Masao Fukuda and Takeshi Fujii},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2008},
  volume={72 3},
  pages={694-701}
}
To understand the response of soil bacteria to the surrounding environment, it is necessary to examine the gene expression profiles of the bacteria in the soil. For this purpose, we developed a new method of extracting RNA from soil reproducibly. Using this new method, we extracted RNA from a field soil, which was sterilized and inoculated with Rhodococcus sp. strain RHA1, a biphenyl degrader isolated from gamma-hexachlorocyclohexane-contaminated soil. Data from agarose gel electrophoresis… CONTINUE READING
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