Detection of bluetongue virus serotype 17 in Culicoides variipennis by nucleic acid blot and sandwich hybridization techniques.

Abstract

Molecular hybridization techniques were developed for the detection and surveillance of bluetongue virus (BTV) serotype 17 in the insect vector Culicoides variipennis, a biting midge. Radiolabeled RNA and cDNA probes were generated from sequences of the L3 segment of BTV serotype 17. These probes were used to detect BTV RNA in pools of infected C… (More)

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@article{Schoepp1990DetectionOB, title={Detection of bluetongue virus serotype 17 in Culicoides variipennis by nucleic acid blot and sandwich hybridization techniques.}, author={R. J. Schoepp and Jeffery Bray and K. E. Olson and Ahmed Mohamed Kamel El-Hussein and F. R. Holbrook and C. D. Blair and P. Roy and B. J. Beaty}, journal={Journal of clinical microbiology}, year={1990}, volume={28 9}, pages={1952-6} }