Detection of base mutations in genomic DNA using denaturing gradient gel electrophoresis (DGGE) followed by transfer and hybridization with gene-specific probes.

Abstract

It has been shown that minor differences, such as single-base-pair substitutions between otherwise identical DNA fragments can result in altered melting behavior detectable by denaturing gradient gel electrophoresis (DGGE). Sequence variations in only a small DNA region within one locus can be detected using the previously described procedures. We have… (More)

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@article{Brresen1988DetectionOB, title={Detection of base mutations in genomic DNA using denaturing gradient gel electrophoresis (DGGE) followed by transfer and hybridization with gene-specific probes.}, author={A. L. B\orresen and Eivind Hovig and A. Br\ogger}, journal={Mutation research}, year={1988}, volume={202 1}, pages={77-83} }