Detection of bacteraemia in critically ill patients using 16S rDNA polymerase chain reaction and DNA sequencing

@article{Sleigh2001DetectionOB,
  title={Detection of bacteraemia in critically ill patients using 16S rDNA polymerase chain reaction and DNA sequencing},
  author={James Wallace Sleigh and Ray T. M. Cursons and Mary La Pine},
  journal={Intensive Care Medicine},
  year={2001},
  volume={27},
  pages={1269-1273}
}
Objective: To confirm the sensitivity of the polymerase chain reaction (PCR) technique (versus blood cultures) and to gain a better understanding of the incidence of true- and false-positive results when using this technique. Design: Observational study. Setting: Fourteen-bed, level 3 intensive care unit. Patients: Hundred twenty-six critically ill adult patients. Hundred ninety-seven blood culture and PCR samples taken as clinically indicated for suspected sepsis, according to routine ICU… CONTINUE READING

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