Detection of autoantibodies in a quantitative immunoassay using recombinant ribonucleoprotein antigens.

@article{Habets1989DetectionOA,
  title={Detection of autoantibodies in a quantitative immunoassay using recombinant ribonucleoprotein antigens.},
  author={W. J. Habets and M H Hoet and Peter T. Sillekens and Dirk Jan de Rooij and LevinusB. A. van de Putte and Walther J. van Venrooij},
  journal={Clinical and experimental immunology},
  year={1989},
  volume={76 2},
  pages={
          172-7
        }
}
A human cDNA expression library was screened with anti-ribonucleoprotein (RNP) antibodies from patients with connective tissue diseases. Three cDNA clones were isolated encoding 70 kD, A and B" ribonucleoprotein autoantigens which were expressed as beta-galactosidase fusion proteins. Antigens were purified and used to develop sensitive ELISAs suitable for the routine screening of large series of sera from patients with connective tissue diseases. More than 400 sera were tested both by ELISA and… CONTINUE READING

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