Detection of atypically light and immunologically defective erythrocytes in patients with paroxysmal nocturnal hemoglobinuria

Centrifugation of defibrinated blood on a density gradient at 600g for 30 min revealed the presence of atypically light erythrocytes, located on the boundary between plasma and Ficoll solution with a specific gravity of 1.077 g/cm3 in 24 of 26 patients with paroxysmal nocturnal hemoglobinuria (PNH). Atypically light erythrocytes are characterized by a… (More)