Detection of antisperm antibodies by immobilization and cytotoxicity tests using flow cytometry

@article{Nikolaeva1992DetectionOA,
  title={Detection of antisperm antibodies by immobilization and cytotoxicity tests using flow cytometry},
  author={M. N. Nikolaeva and M. Sh. Verbitskii and Gennady T. Sukhikh},
  journal={Bulletin of Experimental Biology and Medicine},
  year={1992},
  volume={114},
  pages={1491-1493}
}
Detection of antisperm antibodies (ASAB) is an important stage in the diagnosis of infertility. The most widely used methods of determination of ASAB are those of sperm immobilization [1] or sperm cytotoxicity [2], based on the immobilizing and (or) cytotoxic action of ASAB on spermatozoa, and involving counting the number of immobilized or dead spermatozoa after their incubation in test serum in the presence of complement. However, an important disadvantage of these methods is their… CONTINUE READING

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