Detection of antibodies to ovine lentivirus using recombinant capsid and transmembrane proteins.


The coding sequences of the capsid protein p25 and transmembrane protein of Maedi-Visna virus were amplified using polymerase chain reaction and cloned into the plasmid expression vector pRSET-B. Both DNA constructs expressed proteins tagged with polyhistidine. The recombinant proteins were purified using Ni-NTA agarose and used in immunoblot to detect… (More)


  • Presentations referencing similar topics