Detection of antibodies to Toxoplasma gondii in domesticated ruminants by recombinant truncated SAG2 enzyme-linked immunosorbent assay

@article{Singh2014DetectionOA,
  title={Detection of antibodies to Toxoplasma gondii in domesticated ruminants by recombinant truncated SAG2 enzyme-linked immunosorbent assay},
  author={Harkirat Singh and Anup Kumar Tewari and Ashok Kumar Mishra and Biswaranjan Maharana and Vikrant Sudan and Opinder Krishan Raina and Jammi Raghavendra Rao},
  journal={Tropical Animal Health and Production},
  year={2014},
  volume={47},
  pages={171-178}
}
An antibody detection recombinant enzyme-linked immunosorbent assay (ELISA) specific for Toxoplasma gondii was laboratory standardized using recombinant truncated surface antigen 2 (SAG2) protein of T. gondii. A 483-bp sequence coding for truncated tachyzoite stage-specific SAG2 protein was amplified and ligated in pPROExHT-b expression vector to transform Escherichia coli DH5α cells. A high-level expression of the histidine-tagged fusion protein was obtained after 8 h of incubation. The… CONTINUE READING
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Showing 1-10 of 41 references

Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis

  • E. H. Sawicka, J. Kur, H. Pietkiewicz, L. Holec, A. Gasior, P. Myjak
  • Acta Parasitologica,
  • 2005
Highly Influential
4 Excerpts

Molecular cloning and comparative sequence analysis of open reading frame of SAG2 gene of Toxoplasma gondii

  • H. Singh, A. K. Tewari, A. K. Mishra, B. R. Maharana, J. R. Rao, O. K. Raina
  • Journal of Veterinary Parasitology,
  • 2011
2 Excerpts

Seroprevalence of Toxoplasma gondii in Malaysian cattle

  • W. A. Rahman, V. Manimegalai, P. Chandrawathani, R. Nurulaini, C. M. Zaini, B. Premaalatha
  • Malaysian Journal of Veterinary Research,
  • 2011
1 Excerpt

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