Detection of anionic antimicrobial peptides in ovine bronchoalveolar lavage fluid and respiratory epithelium.

@article{Brogden1998DetectionOA,
  title={Detection of anionic antimicrobial peptides in ovine bronchoalveolar lavage fluid and respiratory epithelium.},
  author={Kim A. Brogden and Martin Ackermann and Kenneth M. Huttner},
  journal={Infection and immunity},
  year={1998},
  volume={66 12},
  pages={5948-54}
}
Three small antimicrobial anionic peptides (AP) were originally isolated from an ovine pulmonary surfactant. However, their presence in bronchoalveolar lavage (BAL) fluid and tissues of the respiratory tract is unknown. In this study, we made affinity-purified rabbit polyclonal and mouse monoclonal antibodies to synthetic H-DDDDDDD-OH. Antibody specificity was assessed by a competitive enzyme-linked immunosorbent assay (ELISA), and the exact epitope binding sites were determined with analog… CONTINUE READING