Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem

@article{Doi2017DetectionOA,
  title={Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem},
  author={Hideyuki Doi and Izumi Katano and Yusuke Sakata and Rio Souma and Toshihiro Kosuge and Mariko Nagano and Kousuke Ikeda and Koki Yano and Koji Tojo},
  journal={Royal Society Open Science},
  year={2017},
  volume={4}
}
The use of environmental DNA (eDNA) has recently been employed to evaluate the distribution of various aquatic macroorganisms. Although this technique has been applied to a broad range of taxa, from vertebrates to invertebrates, its application is limited for aquatic insects such as aquatic heteropterans. Nepa hoffmanni (Heteroptera: Nepidae) is a small (approx. 23 mm) aquatic heteropteran that inhabits wetlands, can be difficult to capture and is endangered in Japan. The molecular tool eDNA… 
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