Detection of amantadine-resistant influenza A virus strains in nursing homes by PCR-restriction fragment length polymorphism analysis with nasopharyngeal swabs.

@article{Saito2002DetectionOA,
  title={Detection of amantadine-resistant influenza A virus strains in nursing homes by PCR-restriction fragment length polymorphism analysis with nasopharyngeal swabs.},
  author={Reiko Saito and Hitoshi Oshitani and Hiroki Masuda and Hiroshi Suzuki},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 1},
  pages={84-8}
}
Annual consumption of amantadine increased abruptly after its approval for the treatment of influenza A virus infections in Japan in 1998, and the emergence of amantadine-resistant viruses is now a matter of concern. To detect resistant influenza A virus strains, we have developed a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis for nasopharyngeal swabs. Three different primer sets for nested PCR were designed to incorporate restriction sites into the amplicon to differentiate… CONTINUE READING

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