Detection of alkaline phosphatase activity after conventional isoelectric focusing by an indoxyl-tetrazolium salt technique.

@article{Sinha1985DetectionOA,
  title={Detection of alkaline phosphatase activity after conventional isoelectric focusing by an indoxyl-tetrazolium salt technique.},
  author={Pranav Sinha},
  journal={Journal of biochemical and biophysical methods},
  year={1985},
  volume={11 6},
  pages={327-40}
}
  • Pranav Sinha
  • Published 1985 in Journal of biochemical and biophysical methods
Alkaline phosphatase was solubilized from human and rat tissues using papain in the presence of TRITON X-100 and subjected to isoelectric focusing (IEF) in polyacrylamide or agarose gels. Up till now, usually 1- and 2-naphthylphosphates have been used as substrates in order to specifically stain molecular forms of this enzyme by the azo-dye technique. In this paper, the use of another histochemical substrate, 5-bromo-4-chloro-3-indoxyl phosphate, in combination with tetrazolium salts [McGadey… CONTINUE READING

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