Detection of aflatoxinogenic fungi in figs by a PCR reaction.

@article{Frber1997DetectionOA,
  title={Detection of aflatoxinogenic fungi in figs by a PCR reaction.},
  author={P. F{\"a}rber and Rolf Geisen and Wilhelm H Holzapfel},
  journal={International journal of food microbiology},
  year={1997},
  volume={36 2-3},
  pages={215-20}
}
A PCR reaction was used to detect aflatoxinogenic Aspergillus flavus strains in contaminated figs. The reaction records the presence of three aflatoxin biosynthesis genes, namely the norsolorinic acid reductase (nor-1), versicolorin A dehydrogenase (ver-1) and sterigmatocystin-o-methyltransferase: (omt-A). The reaction gave a triplet pattern in the presence of DNA from A. flavus isolated from pure cultures. The reaction gave the same PCR products when pure fungal DNA was mixed with pure DNA… CONTINUE READING

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