Detection of adenovirus using PCR and molecular beacon.

  title={Detection of adenovirus using PCR and molecular beacon.},
  author={Saibal K Poddar},
  journal={Journal of virological methods},
  volume={82 1},
The polymerase chain reaction (PCR) and a molecular beacon probe were used for the detection of Adenovirus. A 307 bp DNA fragment from a conserved region of the hexon gene was amplified. The specific molecular beacon was characterized with respect to its efficiency of quenching, and signal to noise ratio by spectrofluorometric analysis of its hybridization with virus specific complementary single stranded oligonucleotide target. Amplification was carried out in the presence of the molecular… CONTINUE READING
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