Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction.

@article{Kuwano1997DetectionOA,
  title={Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction.},
  author={Kazuyoshi Kuwano and Yasushi Nomoto and Ritsuko Kunitake and Naoki Hagimoto and Tokuji Matsuba and Yukihiro Nakanishi and Naoko Hara},
  journal={The European respiratory journal},
  year={1997},
  volume={10 7},
  pages={1445-9}
}
The history of patients with idiopathic pulmonary fibrosis (IPF) shows that the disease may be preceded by a viral-like illness. Although viruses have not been demonstrated, it is possible that viruses were not detected in culture because they do not replicate during latency. We investigated the presence of adenovirus in IPF and interstitial pneumonia associated with collagen vascular disease (CVD-IP), using the nested polymerase chain reaction (PCR) and in situ hybridization (ISH) for the E1A… CONTINUE READING

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