Detection of abasic sites and oxidative DNA base damage using an ELISA-like assay.

@article{Kow2000DetectionOA,
  title={Detection of abasic sites and oxidative DNA base damage using an ELISA-like assay.},
  author={Yoke Wah Kow and Alessandro Dar{\'e}},
  journal={Methods},
  year={2000},
  volume={22 2},
  pages={164-9}
}
Reactive oxygen species produce a wide spectrum of DNA damage, including oxidative base damage and abasic (AP) sites. Many procedures are available for the quantification and detection of base damage and AP sites. However, either these procedures are laborious or the starting materials are difficult to obtain. A biotinylated aldehyde-specific reagent, ARP, has been shown to react specifically with the aldehyde group present in AP sites, resulting in biotin-tagged AP sites in DNA. The biotin… CONTINUE READING

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