Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis.

@article{Clark1999DetectionOA,
  title={Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis.},
  author={Nancye C. Clark and {\O}rjan Olsvik and JanaM. Swenson and Carol A. Spiegel and FredC. Tenover},
  journal={Antimicrobial agents and chemotherapy},
  year={1999},
  volume={43 1},
  pages={
          157-60
        }
}
Genes encoding streptomycin/spectinomycin adenylyltransferases [ANT(3")(9)] have been reported to exist in gram-negative organisms and Staphylococcus aureus. During a study of high-level aminoglycoside resistance in enterococci, we encountered an isolate of Enterococcus faecalis that was streptomycin resistant but did not appear to contain the 6'-adenylyltransferase gene (aadE) when examined by PCR with specific primers. Phosphocellulose paper binding assays indicated the presence of an ANT(3… CONTINUE READING
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