Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

@article{Corman2012DetectionOA,
  title={Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.},
  author={Victor Max Corman and Isabella Anne Eckerle and Tobias Bleicker and Ali Moh Zaki and Olfert Landt and Monika Eschbach-Bludau and Sander van Boheemen and Robin Gopal and Matthias Ballhause and Theo M. Bestebroer and Doreen Muth and M. A. M{\"u}ller and Jan Felix Drexler and Maria Zambon and Albert D.M.E. Osterhaus and R A M Fouchier and Christian Drosten},
  journal={Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin},
  year={2012},
  volume={17 39}
}
  • V. Corman, I. Eckerle, C. Drosten
  • Published 27 September 2012
  • Biology
  • Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We… 
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