Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR.

@article{Keller2010DetectionOA,
  title={Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR.},
  author={P M Keller and Silvana K. Rampini and Guido Vincent Bloemberg},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 6},
  pages={2250-2}
}
We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis. 
6 Citations
12 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 12 references

The expansion of the microbiological spectrum of brain abscesses with use of multiple 16S ribosomal DNA sequencing

  • M. Al Masalma, F. Armougom, +4 authors D. Raoult
  • Clin . Infect . Dis .
  • 2009

The Sanford guide to antimicrobal therapy

  • J. P. Sanford, D. N. Gilbert, R. C. Moellering, G. M. Eliopoulos, M. A. Sande
  • 2008

Direct detection of bacterial pathogens in brain abscesses by polymerase chain reaction amplification and sequencing of partial 16 S ribosomal deoxyribonucleic acid fragments

  • H. Tse
  • 2004

Similar Papers

Loading similar papers…