Detection of a functional xenobiotic response element in a widely employed FoxO-responsive reporter construct.

@article{Eckers2011DetectionOA,
  title={Detection of a functional xenobiotic response element in a widely employed FoxO-responsive reporter construct.},
  author={Anna Eckers and Elisabeth Sauerbier and Anwar Anwar-mohamed and Ingrit Hamann and Charlotte Esser and Peter Schr{\"o}der and Ayman O. S. El-Kadi and Lars-Oliver Klotz},
  journal={Archives of biochemistry and biophysics},
  year={2011},
  volume={516 2},
  pages={
          138-45
        }
}
FHRE-Luc is a promoter reporter construct that is widely used to assess the activity of FoxO (forkhead box, class O) transcription factors. We here demonstrate that this promoter construct responds to exposure of HepG2 human hepatoma cells to known agonists of the aryl hydrocarbon receptor (AhR), 3-methylcholanthrene, benzo(a)pyrene, and 6-formylindolo[3,2-b]carbazole. However, FHRE-Luc activation did not coincide with FoxO DNA binding or changes in Akt-induced FoxO phosphorylation after… CONTINUE READING