Detection of a divergent variant of grapevine virus F by next-generation sequencing

@article{Molenaar2015DetectionOA,
  title={Detection of a divergent variant of grapevine virus F by next-generation sequencing},
  author={N. Molenaar and J. Burger and H. Maree},
  journal={Archives of Virology},
  year={2015},
  volume={160},
  pages={2125-2127}
}
  • N. Molenaar, J. Burger, H. Maree
  • Published 2015
  • Biology, Medicine
  • Archives of Virology
  • The complete genome sequence of a South African isolate of grapevine virus F (GVF) is presented. It was first detected by metagenomic next-generation sequencing of field samples and validated through direct Sanger sequencing. The genome sequence of GVF isolate V5 consists of 7539 nucleotides and contains a poly(A) tail. It has a typical vitivirus genome arrangement that comprises five open reading frames (ORFs), which share only 88.96 % nucleotide sequence identity with the existing complete… CONTINUE READING
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