Detection of a Rod-shaped Virus in Lentinula edodes Mycelium Using RT-PCR

@inproceedings{Lingli2011DetectionOA,
  title={Detection of a Rod-shaped Virus in Lentinula edodes Mycelium Using RT-PCR},
  author={Li Lingli},
  year={2011}
}
Primers designed according to the partial genome sequence(GenBank Accession No: GQ372842) of a rod-shaped virus present in Lentinula edodes mycelium were used to develop a RT-PCR-based viral detection method.Tests conducted on the mycelium of 36 L.edodes cultivars from Zhejiang Province revealed that strains 988,939,937,9319,868,66 and WL-3 were virus-free.Mycelium of 28 other test strains contained the rod-shaped virus but it was unclear if the virus was present in mycelium of L.edodes Ling… CONTINUE READING

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