Detection of a P-glycoprotein related pump in Chironomus larvae and its inhibition by verapamil and cyclosporin A.

@article{Podsiadlowski1998DetectionOA,
  title={Detection of a P-glycoprotein related pump in Chironomus larvae and its inhibition by verapamil and cyclosporin A.},
  author={Lars Podsiadlowski and Vladim{\'i}r Maťha and A. Vilcinskas},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={1998},
  volume={121 4},
  pages={443-50}
}
A membrane associated ATP-dependent efflux pump, similar in function to mammalian P-glycoprotein, was detected in anal papillae of Chironomus riparius larvae. Immunohistochemical analysis of larval tissues, using monoclonal antibodies against P-glycoprotein, was supplemented by functional in vivo and in vitro assays which confirmed the existence of a mechanism for transporting xenobiotic substances. The in vitro ATPase activity of homogenate fractions increased in the presence of typical P… CONTINUE READING