Detection of Yersinia enterocolitica in food by PCR amplification.

@article{Nilsson1998DetectionOY,
  title={Detection of Yersinia enterocolitica in food by PCR amplification.},
  author={Anders T S Nilsson and Susanne Thisted Lambertz and Per St{\aa}lhandske and P. Norberg and Marie-Louise Danielsson-Tham},
  journal={Letters in applied microbiology},
  year={1998},
  volume={26 2},
  pages={
          140-4
        }
}
A polymerase chain reaction (PCR) assay was developed for detection of pathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virF and ail as markers for pathogenicity, detection of species with a virulence factor present was possible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide (CTAB) was followed by two 44 cycle amplification reactions, one for each of the markers. As few as 10(2) Y. enterocolitica cells were detected in ground pork in… CONTINUE READING

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