Detection of Y chromosome-specific DNA in the plasma and urine of pregnant women using nested polymerase chain reaction.

@article{AlYatama2001DetectionOY,
  title={Detection of Y chromosome-specific DNA in the plasma and urine of pregnant women using nested polymerase chain reaction.},
  author={Majda K. Al-Yatama and Abu S Mustafa and Saad Ali and Saji Abraham and Ziauddin Khan and Najmuddin Khaja},
  journal={Prenatal diagnosis},
  year={2001},
  volume={21 5},
  pages={399-402}
}
The present study was undertaken to evaluate a nested polymerase chain reaction (PCR) for detection of Y chromosome-specific fetal DNA in maternal plasma and urine of pregnant women during different gestational stages. DNA isolated from plasma and urine samples of 80 pregnant women (between 7 and 40 weeks' gestation) underwent amplification for Y chromosome-specific 198 bp DNA by nested PCR. The postpartum analysis of fetal gender showed that 55 women carried male and 25 female fetuses. Among… CONTINUE READING

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