Detection of Vibrio cholerae using the intrinsic catalytic activity of a magnetic polymeric nanoparticle.

@article{Thiramanas2013DetectionOV,
  title={Detection of Vibrio cholerae using the intrinsic catalytic activity of a magnetic polymeric nanoparticle.},
  author={Raweewan Thiramanas and Kulachart Jangpatarapongsa and Pramuan Tangboriboonrat and Duangporn Polpanich},
  journal={Analytical chemistry},
  year={2013},
  volume={85 12},
  pages={5996-6002}
}
A novel and sensitive magnetic polymeric nanoparticle (MPNP)-polymerase chain reaction-colorimetry (magneto-PCR-colorimetry) technique was developed for detection of Vibrio cholerae ( V. cholerae ). The technique involved an amplification of V. cholerae DNA on the surface of an MPNP and then employed the intrinsic catalytic activity of the MPNP to detect the target gene by colorimetry. An amino-modified forward primer was covalently labeled onto the MPNP surface which would bind to PCR product… CONTINUE READING