Detection of Toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts

@article{Dumtre2007DetectionOT,
  title={Detection of Toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts},
  author={Aur{\'e}lien Dum{\`e}tre and M L Dard{\'e}},
  journal={Parasitology Research},
  year={2007},
  volume={101},
  pages={989-996}
}
An immunomagnetic separation (IMS) method was developed to detect Toxoplasma gondii in fresh waters by using the monoclonal antibody 4B6 targeting the sporocyst wall of T. gondii, Hammondia hammondi, Hammondia heydorni, and Neospora caninum. Water concentrates obtained by filtering 10- to 20-l samples samples were spiked with Toxoplasma oocysts, sonicated to release the sporocysts, and analyzed by IMS-4B6. Mean sporocyst recoveries were 74.5 ± 5.3% in drinking water, 30.6 ± 2.4 and 37.1 ± 3.2… CONTINUE READING
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Toxoplasmosis of animals and man

  • JP Dubey, CP Beattie
  • 1988
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