Detection of Streptococcus pneumoniae in whole blood by PCR.

  title={Detection of Streptococcus pneumoniae in whole blood by PCR.},
  author={Yi Zhang and Daniel J Isaacman and Robert M. Wadowsky and J Rydquist-White and James Christopher Post and Garth D. Ehrlich},
  journal={Journal of clinical microbiology},
  volume={33 3},
Streptococcus pneumoniae is a major cause of bacteremia in both children and adults. Currently, the diagnosis of pneumococcal bacteremia relies on the isolation and identification of the bacteria from blood cultures. We have developed a sensitive assay for the detection of S. pneumoniae in whole blood by the PCR. A specific primer-probe set (JM201 and JM202 primers with JM204 probe) designed from the penicillin-binding protein 2B gene was demonstrated to reproducibly detect between 10 and 100… CONTINUE READING


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