Detection of Streptococcus pneumoniae and identification of pneumococcal serotypes by real-time polymerase chain reaction using blood samples from Italian children ≤ 5 years of age with community-acquired pneumonia.

@article{Marchese2011DetectionOS,
  title={Detection of Streptococcus pneumoniae and identification of pneumococcal serotypes by real-time polymerase chain reaction using blood samples from Italian children ≤ 5 years of age with community-acquired pneumonia.},
  author={Anna Marchese and Susanna Esposito and Erika Coppo and Giovanni A Rossi and Alberto E. Tozzi and Mariateresa Romano and Liviana da Dalt and Gian Carlo Schito and Nicola Principi},
  journal={Microbial drug resistance},
  year={2011},
  volume={17 3},
  pages={419-24}
}
Streptococcus pneumoniae is a leading cause of severe life-threatening infections. Laboratory identification and serotyping of this pathogens is desirable to monitor vaccine impact and coverage; however, especially in pediatric patients, the yield of traditional microbiological diagnostic procedures can be very low. The aim of this study was to develop real-time polymerase chain reaction (PCR)-based assays to be performed directly on blood samples to identify the most common capsular serotypes… CONTINUE READING
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