Detection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countries

@inproceedings{Selva2013DetectionOS,
  title={Detection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countries},
  author={Laura Selva and Rachid Benmessaoud and Miguel Lanaspa and Imane Jroundi and Cinta Moraleda and Sozinho {\'A}cacio and Melania I{\~n}igo and Alien Bastiani and Manuel Monson{\'i}s and Rom{\'a}n Pallar{\'e}s and Quique Bassat and Carmen Mu{\~n}oz-Almagro},
  booktitle={PloS one},
  year={2013}
}
BACKGROUND Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. METHODS Ply and wzg genes of S… CONTINUE READING
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