Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples.

@article{Hatta2007DetectionOS,
  title={Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples.},
  author={Mochammad Hatta and Henk L. Smits},
  journal={The American journal of tropical medicine and hygiene},
  year={2007},
  volume={76 1},
  pages={139-43}
}
A nested polymerase chain reaction (PCR) specific for Salmonella enterica serovar Typhi was used for the detection of the pathogen in blood, urine, and stool samples from 131 patients with clinical suspicion of typhoid fever. The sensitivity of blood culture, the PCRs with blood, urine, and feces, and the Widal test were 61.8%, 84.5%, 69.3%, 46.9%, and 39.0%, respectively. The sensitivity of the PCRs with blood (P < 0.001) and urine (P = 0.01) were significantly higher, and the sensitivity of… CONTINUE READING

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