• Biology, Medicine
  • Published in
    The Southeast Asian journal…
    2010

Detection of Salmonella invA gene in shrimp enrichment culture by polymerase chain reaction.

@article{Upadhyay2010DetectionOS,
  title={Detection of Salmonella invA gene in shrimp enrichment culture by polymerase chain reaction.},
  author={Bishnu Prasad Upadhyay and Fuangfa Utrarachkij and Jarinee Thongshoob and Yuvadee Mahakunkijcharoen and Niracha Wongchinda and Orasa Suthienkul and Srisin Khusmith},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={2010},
  volume={41 2},
  pages={
          426-35
        }
}
Contamination of seafood with salmonellae is a major public health concern. Detection of Salmonella by standard culture methods is time consuming. In this study, an enrichment culture step prior to polymerase chain reaction (PCR) was applied to detect 284 bp fragment of Salmonella invA in comparison with the conventional culture method in 100 shrimp samples collected from four different shrimp farms and fresh food markets around Bangkok. Samples were pre-enriched in non-selective lactose broth… CONTINUE READING

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