Detection of Salmonella in environmental water and sediment by a nested-multiplex polymerase chain reaction assay.

@article{Touron2005DetectionOS,
  title={Detection of Salmonella in environmental water and sediment by a nested-multiplex polymerase chain reaction assay.},
  author={Aur{\'e}lie Touron and Thierry Berthe and Barbara Pawlak and Fabienne Petit},
  journal={Research in microbiology},
  year={2005},
  volume={156 4},
  pages={541-53}
}
From 1995 to 2002, 53 serovars of Salmonella were isolated in the Seine estuary (France). The 3 serovars most frequently found were S. enterica serovar Typhimurium, S. enterica serovar Infantis and S. enterica serovar Virchow. A nested multiplex PCR (nm-PCR) assay was developed to detect the presence of Salmonella in estuarine water and sediment samples. The target gene used was the phase 1 flagellin fliC chromosomal gene, present in all Salmonella serovars. A set of 4 primers was first used to… CONTINUE READING

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