Detection of SARS-Coronavirus-2 in wastewater, using the existing environmental surveillance network: An epidemiological gateway to an early warning for COVID-19 in communities.

@article{Sharif2020DetectionOS,
  title={Detection of SARS-Coronavirus-2 in wastewater, using the existing environmental surveillance network: An epidemiological gateway to an early warning for COVID-19 in communities.},
  author={Salmaan Sharif and Aamer Ikram and Adnan Khurshid and Muhammad Salman and Nayab Mehmood and Yasir Arshad and Jamal Ahmad and Rana M Safdar and Mehar Angez and Muhammad Masroor Alam and Lubna Rehman and Ghulam Mujtaba and Jaffar Ap Hussain and Johar Ali and Ribqa Akthar and Muhammad Wasif Malik and Zeeshan Iqbal Baig and Muhammad Suleman Rana and Muhammad Usman and Muhammad Qaisar Ali and Abdul Ahad and Nazish Badar and Massab Umair and Sana Tamim and Asiya Ashraf and Faheem Tahir and Nida Ali},
  journal={medRxiv},
  year={2020}
}
The ongoing COVID-19 pandemic caused by SARs-CoV-2 was considered to be transmitted person to person via droplet infections and fecal-oral transmission. To determine this, we used the existing polio environment surveillance network in Pakistan to investigate presence of SARs-CoV-2 using three commercially available kits and E-Gene detection published assay for surety and confirmatory of positivity. A Two-phase separation method is used for sample clarification and concentration. Before… 
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