Detection of Pneumocystis jirovecii by nested PCR in HIV-negative patients with pulmonary disease.

BACKGROUND Nested PCR can be used to determine the status of Pneumocystis jirovecii infection in other lung diseases. AIMS This study sought to detect a target DNA fragment (mitochondrial large subunit rRNA or mtL SUrRNA) of P. jirovecii in patients with lung disease who underwent bronchoscopy with collection of bronchoalveolar lavage (BAL). METHODS The… CONTINUE READING