Detection of Phytoplasmas Causing Grassy Shoot Disease in Sugarcane by PCR Technique

  title={Detection of Phytoplasmas Causing Grassy Shoot Disease in Sugarcane by PCR Technique},
  author={Rasappa Viswanathan and M. Balamuralikrishnan and Muthusamy Poongothai},
  journal={Sugar Tech},
Polymerase chain reaction technique was standardized for the detection of phytoplasmas causing grassy shoot disease (GSD) in sugarcane using two sets of forward and reverse primers of 16S rRNA/23S spacer region and 16S of rRNA were specifically amplified by PCR, respectively using these universal primers from the DNA of the GSD-affected plants. The results indicate that PCR based diagnosis can be used for the detection of GSD phytoplasmas in sugarcane. 


Publications referenced by this paper.

Grassy shoot

P. Wongkeaw, Y. Hanboonsong, +4 authors S. Damak
  • 2000

Complex phytoplasma flora in Northeast Thailand as revelaed by 16 S rDNA analysis

S. Namba, S. Kato
  • Ann . Phytopathol . Soc . Japan
  • 1996

Phylogtenetic relationships of plant pathogenic MLO established by 16 s / 23 s spacersequences

I. M. Lee, R. W. Hammond, R. E. Davis, D. E. Gundersen
  • IOM Letter
  • 1994

Universla amplification and analysis of pathogen 16 s rDNA for classification and identification of mycoplasma like organisms

C. Mareone, A. Ragozzino, E. Seemuller
  • Phytopathology .
  • 1993

Amplification of 16 S rRNA genes from culturable and nonculturable Molicutes

S. Edison, K. Ramakrishnan, P. Narayanasamy
  • J . Microbiol . Meth .
  • 1991

Grass shoot and white leaf disease

B. C. Ricaud, A. G. Gillaspie, C. G. Hughes, R. Sdoodee
  • 1989

Similar Papers

Loading similar papers…