Detection of Phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction

@article{NagendraPrasad2009DetectionOP,
  title={Detection of Phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction},
  author={M. N. Nagendra Prasad and S. Shankara Bhat and A. P. Charith Raj and G. Ranga Janardhana},
  journal={Archives of Phytopathology and Plant Protection},
  year={2009},
  volume={42},
  pages={124 - 128}
}
Abstract Twigs and seeds of Azadirachta indica (Neem) infected with dieback disease, collected from different regions of Karnataka, India were analysed to determine the pathogens. Phomopsis azadirachtae, the causal agent of dieback disease of neem was found to be seed borne. Phomopsis azadirachtae was isolated on PDA and MEA from dieback-infected neem twigs, seeds and embryo. Phomopsis genus-specific primers (5.8S r-DNA) were then used for the detection of Phomopsis azadirachtae, the causative… 
Study of die back disease incidence of neem in Karnataka, India and PCR based identification of the isolates
TLDR
A disease survey of die back of neem was done in different agroclimatic regions of Karnataka, India using Global Positioning System (GARMIN 12) to determine the pathogen and isolated P. azadirachtae, the causative agent of death back disease on neem from diseased trees.
A survey of die-back disease of neem in Tamil Nadu, India and PCR-based confirmation of the isolates
TLDR
A survey of die-back disease of neem was done in different agro climatic regions of Tamil Nadu, India using Global Positioning System (GARMIN 12) to determine the pathogen and isolated P. azadirachtae from the diseased trees confirming the causal organism.
Development of a simple and reliable species-specific detection of Phomopsis azadirachtae, using the translation elongation factor 1-alpha gene
TLDR
A nested PCR protocol was designed which proved efficient in detection of the pathogen in neem twigs obtained by artificial inoculation one day after infection, with the nested PCR method, and the results provide a highly sensitive method in early and rapid detection of PA and facilitate in primary screening of the infection.
Stem canker and dieback disease on Grevillea robusta Cunn ex R. Br.
TLDR
Analysis of morphological characteristics and molecular data of the internal transcribed spacer (ITS) rDNA revealed that forty fungal species were associated with G. robusta, a widespread stem canker and dieback disease threatened the cultivation of Grevillea robusta on farms in Kenya.
In vitro efficacy of plant essential oils against Phomopsis azadirachtae – the causative agent of die-back disease of neem
TLDR
Seven essential oils were used and Palmarosa proved to be most potent showing complete inhibition of mycelia at 500 ppm, which really suggests that plant sources possess antifungal activity and can be effectively used to treat and manage plant diseases thereby circumventing the fungal spread in the authors' environment.
eJBio: Electronic Journal of Biology
TLDR
A new fungus Phomopsis azadirachtae was reported on neem causing dieback, a native tree to India, which results in almost 100% loss of fruit production.
Antifungal activity of essential oils against Phomopsis azadirachtae - the causative agent of die-back disease of neem
TLDR
The results of the present investigations indicate that plant essential oils possess antifungal activity and can be exploited as an ideal treatment for future plant disease management to eliminate fungal spread.
Overlooked competing asexual and sexually typified generic names of Ascomycota with recommendations for their use or protection
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Recommendations for additional competing genera not considered in previous papers for use or protection of competing generic names in major groups of ascomycetes are made.

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TLDR
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Basic plant pathology methods
Basic plant pathology methods , Basic plant pathology methods , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
National Research Council
The National Research Council has elected the following chairmen of its various divisions for the year beginning July 1, 1920:
Molecular detection of Phomopsis
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