Detection of Papaya Ringspot Virus by Immuno-capture PCR, Nested-PCR and ELISA-PCR Assays

@inproceedings{Huo2000DetectionOP,
  title={Detection of Papaya Ringspot Virus by Immuno-capture PCR, Nested-PCR and ELISA-PCR Assays},
  author={Xiao Ping Huo},
  year={2000}
}
Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 5×10 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively. 

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Ultra-sensitive detection of papaya ringspot virus using single-tube nested PCR.

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