Detection of PCR products using self-probing amplicons and fluorescence

  title={Detection of PCR products using self-probing amplicons and fluorescence},
  author={David Whitcombe and Jane Theaker and Simon P. Guy and Tom Brown and Steve Little},
  journal={Nature Biotechnology},
Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays, and a number of techniques rely on energy transfer between a fluorophore and a proximal quencher molecule. In these methods, dual-labeled probes hybridize to an amplicon and changes in the quenching of the fluorophore are detected. We describe a new technology that is simple… CONTINUE READING
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