Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in Unengorged Chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by Nested Polymerase Chain Reaction

@inproceedings{Pham2001DetectionOO,
  title={Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in Unengorged Chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by Nested Polymerase Chain Reaction},
  author={X. Pham and Y. Otsuka and H. Suzuki and H. Takaoka},
  booktitle={Journal of medical entomology},
  year={2001}
}
Abstract The current study surveyed the 56-kDa type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi (Hayashi) in ≈4,000 unengorged chiggers obtained from the soil or ground surface in an endemic and a nonendemic area of the Tsutsugamushi disease in Oita Prefecture, southwestern Japan, by nested polymerase chain reaction (PCR). Serotypes of O. tsutsugamushi were identified by restriction fragment-length polymorphism (RFLP) analysis. In the endemic area, 242 pools from five species… Expand
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To identify potential vector species of scrub typhus in the Republic of Korea (ROK), chigger mites were harvested from wild rodents captured at nine localities in October 2005 and examined for Orientia tsutsugamushi DNA by nested polymerase chain reaction. Expand
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TLDR
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