Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in Unengorged Chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by Nested Polymerase Chain Reaction

@inproceedings{Pham2001DetectionOO,
  title={Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in Unengorged Chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by Nested Polymerase Chain Reaction},
  author={Xuan Da Pham and Yasushi Otsuka and H. Suzuki and Hiroyuki Takaoka},
  booktitle={Journal of medical entomology},
  year={2001}
}
Abstract The current study surveyed the 56-kDa type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi (Hayashi) in ≈4,000 unengorged chiggers obtained from the soil or ground surface in an endemic and a nonendemic area of the Tsutsugamushi disease in Oita Prefecture, southwestern Japan, by nested polymerase chain reaction (PCR). Serotypes of O. tsutsugamushi were identified by restriction fragment-length polymorphism (RFLP) analysis. In the endemic area, 242 pools from five species… 
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TLDR
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TLDR
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