Detection of Newcastle Disease Virus in Infected Chicken Embryos and Chicken Tissues by Rt-pcr

  • KRZYSZTOF, MIETANKA, +2 authors SKA-BLICHARZ
  • Published 2006
RT-PCR for the detection of Newcastle disease virus (NDV) in allantoic fluids of SPF embryonated eggs as well as in tissues of SPF chickens infected experimentally is described. The method proved to be specific as all tested NDVs were detected and no cross reaction with other RNA viruses was observed. Sensitivity of the method was established at 10ELD50/0.1… CONTINUE READING