Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay
@inproceedings{Berniak2013DetectionON, title={Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay}, author={H. Berniak and B. Komorowska and D. Sochacki}, year={2013} }
Abstract A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV isolates, the conserved sequence fragments were identified in viral polyprotein gene and 3’UTR region. Based on those fragments, a forward NLs1 and two reverse: NLa1 and NLSCPR2 primers were designed. Primer pairs NLs1-NLa1 and NLs1- NLSCPR2 proved to be effective in amplification of a 669 bp and 1295 bp products, respectively… CONTINUE READING
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References
SHOWING 1-10 OF 20 REFERENCES
A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family
- Biology, Medicine
- Archives of Virology
- 2001
- 212
Narcissus latent, a virus with filamentous particles and a novel combination of properties
- Biology
- 1991
- 12
Silicacapture-Reverse Transcription-Polymerase Chain Reaction (SC-RT-PCR): Application for the Detection of Several Plant Viruses
- Chemistry
- 1997
- 31
Comparison of three techniques for detection of grapevine leafroll-associated virus 1.
- Biology, Medicine
- Acta virologica
- 2005
- 8
Some hosts and properties of narcissus latent virus, a carlavirus commonly infecting narcissus and bulbous iris.
- Biology
- 1977
- 19
- Highly Influential
Characterisation of polyclonal antibodies raised against two isolates of cucumber mosaic virus
- Biology
- 2010
- 8
- PDF
Rapid and simple method for purification of nucleic acids.
- Biology, Medicine
- Journal of clinical microbiology
- 1990
- 4,954
- PDF