Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay

@inproceedings{Berniak2013DetectionON,
  title={Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay},
  author={Hanna Berniak and Beata Komorowska and Dariusz Sochacki},
  year={2013}
}
Abstract A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV isolates, the conserved sequence fragments were identified in viral polyprotein gene and 3’UTR region. Based on those fragments, a forward NLs1 and two reverse: NLa1 and NLSCPR2 primers were designed. Primer pairs NLs1-NLa1 and NLs1- NLSCPR2 proved to be effective in amplification of a 669 bp and 1295 bp products, respectively… 

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References

SHOWING 1-10 OF 21 REFERENCES

A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family

TLDR
A universal primer, designed from the consensus sequences that code for the conserved sequence GNNSGQP in the NIb region of members of the family Potyviridae, was used to amplify by RT-PCR the 3′-terminal genome regions from infected plant samples representing 21 different viruses in the family.

Narcissus latent, a virus with filamentous particles and a novel combination of properties

TLDR
Narcissus latent and narcissus yellow stripe viruses seem to be synonymous and, together with MMV, have properties distinct from those of any previously described virus group.

Silicacapture-Reverse Transcription-Polymerase Chain Reaction (SC-RT-PCR): Application for the Detection of Several Plant Viruses

TLDR
Application of polymerase chain reaction (PCR) for the detection of plant RNA viruses is considerably limited by problems with the first step - synthesis of cDNA complementary to RNA of pathogen present in the infected tissue.

Comparison of three techniques for detection of grapevine leafroll-associated virus 1.

Thirty seven plants of grapevine from the Research Station of Viticulture, Karlstejn was examined for the presence of leafroll viruses. Grapevine leafroll-associated virus 1 (GLRaV-1) was detected in

Five viruses in Narcissus spp. from New Zealand

Narcissus spp., showing virus-like symptoms from Otago Province New Zealand, were surveyed for viruses using ELISA and mechanical transmission tests. High incidence of virus infection was detected at

Characterisation of polyclonal antibodies raised against two isolates of cucumber mosaic virus

TLDR
The suitability of a mixture of PAbs for immunocapturing CMV and for the sensitive detection of all viral isolates tested by IC-RTPCR was also shown.

Diagnosis of plant viral pathogens

TLDR
Current diagnosis techniques are broadly divided into serological procedures, nucleic acid procedures and combinations of both, and upcoming methods are presented.

Control situation of virus diseases in narcissus in the Netherlands

TLDR
The control situation of virus diseases in narcissi grown in the Netherlands was studied and the elimination of high virus-source rates by the roguing of plants is amply applied to reduce virus spread indirectly.

Rapid and simple method for purification of nucleic acids

TLDR
A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.

Virus and virus-like diseases of bulb and flower crops

TLDR
The economic importance of Ornamentals and Pot and Bedding Plants and Viruses, and VIRUS DISEASes of MAJOR ORNAMENTAL CROPS.