Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.

@article{Rindi2004DetectionOM,
  title={Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.},
  author={Laura Rindi and Nicoletta Lari and Daniela Bonanni and Carlo Garzelli},
  journal={Journal of microbiological methods},
  year={2004},
  volume={59 2},
  pages={283-7}
}
A duplex real-time PCR assay was developed for the assignment of Mycobacterium tuberculosis isolates to the three genotypic groups based on the katG463/gyrA95 polymorphism. The assay was as sensitive and specific as nucleotide sequencing and proved also able to detect unambiguously the isolate genotype in clinical specimens.