Detection of Mycobacterium avium subsp paratuberculosis in formalin-fixed paraffin-embedded intestinal tissue by IS900 polymerase chain reaction.

Abstract

OBJECTIVES To evaluate and compare methods for DNA extraction from formalin-fixed, paraffin-embedded tissues and methods for detection of Mycobacterium avium subsp paratuberculosis by IS900 PCR for confirmation of Johne's disease in ruminants. DESIGN A laboratory study. PROCEDURE Three methods of DNA extraction of differing complexity and two PCR… (More)

Topics

  • Presentations referencing similar topics